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Title of Journal: Arch Virol

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Abbravation: Archives of Virology

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Springer Vienna

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DOI

10.1016/0002-9149(68)90052-0

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ISSN

1432-8798

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Molecular detection of genotype 3 porcine hepatiti

Authors: Myra Hosmillo YoungJu Jeong HyunJeong Kim JunGyu Park Mukti Kant Nayak Mia Madel Alfajaro Therese Marie Collantes SuJin Park Kazuyoshi Ikuta Mikihiro Yunoki MunIl Kang SangIk Park KyoungOh Cho
Publish Date: 2010/05/11
Volume: 155, Issue: 7, Pages: 1157-1161
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Abstract

By reverse transcription RTnested polymerase chain reaction PCR hepatitis E virus HEV RNA was simultaneously detected in the livers of aborted fetuses and in fecal and serum samples from their sows obtained from two of 14 farms tested Sequence and phylogenetic analyses showed that these HEVs belonged to genotype 3 HEV HEV RNA was detected by realtime RTPCR at 20 × 103–28 × 104 viral copies/μl in the HEVpositive samples All HEVpositive fetuses tested positive for porcine circovirus type 2 PCV2 These results indicate that transplacental infection of HEV with PCV2 coinfection may occur in sows with reproductive failure which is suggestive of similarities to HEV infection in womenThis study was supported by grant 2009–0081752 from the Korea Science and Engineering Foundation KOSEF and the Regional Technology Innovation Program of the Ministry of Commerce Industry and Energy MOCIE Republic of Korea The authors would like to acknowledge a graduate fellowship from the Brain Korea 21 project of the Korean Ministry of Education and Human Resources Development

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