Authors: D Pramesh Bikash Mandal Chigurupati Phaneendra V Muniyappa
Publish Date: 2012/10/25
Volume: 158, Issue: 3, Pages: 531-542
Abstract
Croton yellow vein mosaic virus CYVMV is a widely occurring begomovirus in Croton bonplandianum a common weed in the Indian subcontinent In this study CYVMV genus Begomovirus family Geminiviridae was transmitted by whiteflies Bemisia tabaci to as many as 35 plant species belonging to 11 families including many vegetables tobacco varieties ornamentals and weeds CYVMV produced bright yellow vein symptoms in croton whereas in all the other host species the virus produced leaf curl symptoms CYVMV produced leaf curl in 13 tobacco species and 22 cultivars of Nicotiana tabacum and resembled tobacco leaf curl virus TobLCV in host reactions However CYVMV was distinguished from TobLCV in four differential hosts Ageratum conyzoides C bonplandianum Euphorbia geniculata and Sonchus bracyotis The complete genome sequences of four isolates originating from northern eastern and southern India revealed that a single species of DNAA and a betasatellite croton yellow vein mosaic betasatellite CroYVMB were associated with the yellow vein mosaic disease of croton The sequence identity among the isolates of CYVMV DNAA and CroYVMB occurring in diverse plant species was 918979 and 833100 respectively The CYVMV DNAA and CroYVMB generated through rollingcircle amplification of the cloned DNAs produced typical symptoms of yellow vein mosaic and leaf curling in croton and tomato respectively The progeny virus from both the croton and tomato plants was transmitted successfully by B tabaci The present study establishes the etiology of yellow vein mosaic disease of C bonplandianum and provides molecular evidence that a weedinfecting monopartite begomovirus causes leaf curl in tomatoA fellowship provided by the Indian Council for Agricultural Research ICAR New Delhi India and Postgraduate School Indian Agricultural Research Institute IARI New Delhi India to the first author is thankfully acknowledged We thank Neha Tiwari for her help in biolistic inoculation We are thankful to Dr MN Maruthi Natural Resources Institute University of Greenwich UK for critically reviewing the manuscript
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