Authors: WenKang Liu XiangYang Jiang ZhenXi Zhang
Publish Date: 2010/03/13
Volume: 155, Issue: 5, Pages: 657-663
Abstract
The purpose of our study was to investigate the expression of prostate stem cell antigen PSCA piwilike 1 PIWIL1 and Tbox 2 TBX2 and its correlation with HPV16 infection in cervical squamous cell carcinoma CSCC HPV16 was detected by amplifying the HPV16 E7 gene by the polymerase chain reaction PCR method and the expression of PSCA PIWIL1 TBX2 and HPV16 E7 in 59 CSCCs and matched adjacent normal cervix MANC was examined by the streptavidinperoxidase SP method Fiftytwo CSCCs and MANC specimens that were positive for the E7 gene and the E7 protein were identified as infected with HPV16 and included in present study The rate of infection with HPV16 in CSCC was 52 27/52 but that in matched adjacent normal cervix MANC samples was 4 2/52 Infection with HPV16 was found to be statistically more frequent in CSCC P = 0000 The expression rates of PSCA PIWIL1 and TBX2 in MANC were 6 3/52 8 4/52 and 2 1/52 respectively but those in CSCC were 62 32/52 75 39/52 and 52 27/52 respectively Higher expression rates of PSCA PIWIL1 and TBX2 were observed in CSCC than in MANC P = 0000 HPV16 had a statistical positive correlation with PSCA PIWIL1 and TBX2 in CSCC P 005 The increased expression of PSCA PIWIL1 and TBX2 had no correlation with the patient’s age or histological grade P 005 The elevated expression of PSCA and PIWIL1 was associated with invasion of CSCC P 005 Upregulated expression of TBX2 had a positive association with lymph node metastasis P = 0014 These findings demonstrate for the first time the expression of PSCA PIWIL1 and TBX2 in CSCC Their correlation with HPV16 might provide new basic information for investigating the molecular mechanism of HPV and help us to deepen our understanding of the interaction between HPV16 and host cells the carcinogenesis of CSCC
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