Authors: Joana M S Cardoso Maria R Félix Maria I E Clara Solange Oliveira
Publish Date: 2009/06/09
Volume: 154, Issue: 7, Pages: 1169-1172
Abstract
The first complete genome sequence of an isolate of Tobacco necrosis virusD TNVD genus Necrovirus family Tombusviridae was that of a bean isolate from England 4 The complete nucleotide sequence of another TNVD isolate an Hungarian isolate from tobacco plants TNVDH revealed a similar genomic organization 11 The singlestranded positivesense RNA genome of TNVD is about 38 kb in size and contains six open reading frames ORFs The two 5′proximal ORFs are likely to be translated from the same initiation codon by translational readthrough of the amber stop codon allowing the synthesis of the putative RNAdependent RNA polymerase RdRp For the TNVDH genome three small ORFs were deduced located between the ORFs encoding the putative RdRp and the other major translation product the coat protein CP encoded at the 3′ end These small ORFs encode three different 7kDa proteins p71 p7a and p7b
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