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Title of Journal: Arch Virol

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Abbravation: Archives of Virology

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Springer Vienna

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DOI

10.1007/s12263-015-0482-2

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ISSN

1432-8798

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Antiviral activity of recombinant porcine surfacta

Authors: Lan Li Qisheng Zheng Yuanpeng Zhang Pengcheng Li Yanfeng Fu Jibo Hou Xilong Xiao
Publish Date: 2016/04/21
Volume: 161, Issue: 7, Pages: 1883-1890
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Abstract

Porcine reproductive and respiratory syndrome virus PRRSV has caused significant economic losses in the swine industry worldwide However there is not an ideal vaccine to provide complete protection against PRRSV Thus the need for new antiviral strategies to control PRRSV still remains Surfactant protein A SPA belongs to the family of Ctype lectins which can exert antiviral activities In this present study we assessed the antiviral properties of recombinant porcine SPA RpSPA on PRRSV infection in Marc 145 cells and revealed its antiviral mechanism using a plaque assay realtime qPCR western blotting analysis and an attachment and penetration assay Our results showed that RpSPA could inhibit the infectivity of PRRSV in Marc 145 cells and could reduce the total RNA and protein level The attachment assay indicated that RpSPA in the presence of Ca2+ could largely inhibit Marc 145 cell attachment however in the penetration assay it was relatively inactive Furthermore our study suggested that virus progeny released from infected Marc145 cells were blocked by RpSPA from infecting other cells We conclude that RpSPA has antiviral activity against PRRSV most probably by blocking viral attachment and the celltocell transmission pathway and therefore RpSPA holds promise as a novel antiviral agent against PRRSV

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