Authors: Mabel Rodríguez Christopher Wood Rosana SanchezLópez Ricardo M CastroAcosta Octavio T Ramírez Laura A Palomares
Publish Date: 2013/11/15
Volume: 159, Issue: 5, Pages: 1005-1015
Abstract
Rotavirus VP6 nanotubes are an attractive option for a recombinant vaccine against rotavirus disease Protection against rotavirus infection and an adjuvant effect have been observed upon immunization with VP6 nanotubes However little information exists on how VP6 nanotubes interact with cells and trigger an immune response In this work the interaction between VP6 nanotubes and different cell lines was characterized VP6 nanotubes were not cytotoxic to any of the animal or human cell lines tested Uptake of nanotubes into cells was celllinedependent as only THP1 and J774 macrophage cells internalized them Moreover the size and spatial arrangement of VP6 assembled into nanotubes allowed their uptake by macrophages as doublelayered rotaviruslike particles also displaying VP6 in their surface were not taken up The internalization of VP6 nanotubes was inhibited by methylβcyclodextrin but not by genistein indicating that nanotube entry is specific depends on the presence of cholesterol in the plasma membrane and does not require the activity of tyrosine kinases The information generated here expands our understanding of the interaction of protein nanotubes with cells which is useful for the application of VP6 nanotubes as a vaccineFinancial support was provided by SEPConacyt 101847 and PAPIITUNAM IN223210 and IT200113 MR received financial support from Conacyt during her graduate studies Technical support was provided by A R Pastor and V Hernández and by G Zavala and A N Lecona at the Electron Microscopy Units of Instituto de Biotecnología UNAM and Instituto Nacional de Salud Pública Mexico and J A Pimentel at the Laboratorio Nacional de Microscopía Avanzada UNAM México Materials were provided by Dr Y Rosenstein Dr A Valdez and Dr C Treviño
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