Authors: Tetsuya Yoshida Yugo Kitazawa Ken Komatsu Yutaro Neriya Kazuya Ishikawa Naoko Fujita Masayoshi Hashimoto Kensaku Maejima Yasuyuki Yamaji Shigetou Namba
Publish Date: 2014/07/15
Volume: 159, Issue: 11, Pages: 3161-3165
Abstract
In this study we detected a Japanese isolate of hibiscus latent Fort Pierce virus HLFPVJ a member of the genus Tobamovirus in a hibiscus plant in Japan and determined the complete sequence and organization of its genome HLFPVJ has four open reading frames ORFs each of which shares more than 98 nucleotide sequence identity with those of other HLFPV isolates Moreover HLFPVJ contains a unique internal polyA region of variable length ranging from 44 to 78 nucleotides in its 3′untranslated region UTR as is the case with hibiscus latent Singapore virus HLSV another hibiscusinfecting tobamovirus The length of the HLFPVJ genome was 6431 nucleotides including the shortest internal polyA region The sequence identities of ORFs 1 2 3 and 4 of HLFPVJ to other tobamoviruses were 466–687 499–708 310–708 and 394–701 respectively at the nucleotide level and 398–750 436–778 192–704 and 312–742 respectively at the amino acid level The 5′ and 3′UTRs of HLFPVJ showed 243–586 and 130–798 identity respectively to other tobamoviruses In particular when compared to other tobamoviruses each ORF and UTR of HLFPVJ showed the highest sequence identity to those of HLSV Phylogenetic analysis showed that HLFPVJ other HLFPV isolates and HLSV constitute a malvaceousplantinfecting tobamovirus cluster These results indicate that the genomic structure of HLFPVJ has unique features similar to those of HLSV To our knowledge this is the first report of the complete genome sequence of HLFPV
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