Authors: Xiao Wang Feifei Meng Dandan Wang Xing Liu Sujuan Chen Tao Qin Daxin Peng Xiufan Liu
Publish Date: 2016/09/02
Volume: 161, Issue: 12, Pages: 3365-3374
Abstract
Novel reassortant influenza A H5N8 viruses are becoming a potential threat not only to the poultry industry but also to public health Many molecular markers for pathogenicity in mammalian hosts have been identified in other H5 subtype avian influenza viruses AIVs However the pathogenicity of H5N8 AIVs to mammals remains unclear It is believed that selection of a pair of isolates with a similar genetic background but with different virulence to mammals is a prerequisite for studying the pathogenic mechanism of AIVs Two avianorigin H5N8 isolates A/goose/Eastern China/CZ/2013 CZ13 and A/duck/ Eastern China /JY/2014 JY14 which shared a similar genetic background H5 clade 2344 and amino acid substitutions that were shown previously to be molecular markers of pathogenicity were used to determine their biological characteristics and pathogenicity Hemagglutination assays using α23sialidasetreated goose red blood cells demonstrated that both viruses exhibited a dualreceptorbinding preference Viral growth kinetics in vitro indicated that both viruses replicated to high titers in CEF cells about 1080 TCID50/mL In MDCK cells however CZ13 replicated efficiently 1070 TCID50/mL while JY14 grew to peak titers below 1040 TCID50/mL Animal studies indicated that although both viruses were highly virulent in chickens they exhibited significantly different virulence in mice CZ13 was highly pathogenic MLD50 = 1016EID50 whereas JY14 had low virulence MLD50 1065 EID50 Therefore this pair of viruses can be used to search for unknown molecular markers of virulence and to investigate specific pathogenic mechanisms in miceThis work was supported by the National Natural Science Foundation of China Grant Number 31372450 31402229 the Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province Grant Number CX151065 the Sanxin Engineering Project for Jiangsu Agriculture Grant Number SXGC2014311 the Yangzhou University Funding for Scientific Research Grant Number KYLX15 1380 the National key RD Project Grant Number 2016YFD0500202 and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education InstitutionsThe Jiangsu Administrative Committee for Laboratory Animals approved all animal studies Permit Number SYXKSU20070005 according to the guidelines of Jiangsu Laboratory Animal Welfare and Ethical of Jiangsu Administrative Committee of Laboratory Animals
Keywords: