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Title of Journal: Arch Virol

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Abbravation: Archives of Virology

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Springer Vienna

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DOI

10.1007/bf01134236

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ISSN

1432-8798

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Development and evaluation of a recombinantglycop

Authors: Ebenezer Angel Jemima Seeralan Manoharan Kathaperumal Kumanan
Publish Date: 2014/03/04
Volume: 159, Issue: 8, Pages: 1987-1993
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Abstract

The measurement of neutralizing antibodies induced by the glycoprotein of rabies virus is indispensable for assessing the level of neutralizing antibodies in animals or humans A rapid fluorescent focus inhibition test RFFIT has been approved by WHO and is the most widely used method to measure the virusneutralizing antibody content in serum but a rapid test system would be of great value to screen large numbers of serum samples To develop and evaluate a latex agglutination test LAT for measuring rabies virus antibodies a recombinant glycoprotein was expressed in an insect cell system and purified and the protein was coated onto latex beads at concentrations of 01 025 05 075 and 1 mg/ml to find out the optimal concentration for coating latex beads It was found that 05 mg/ml of recombinant protein was optimal for coating latex beads and this concentration was used to sensitize the latex beads for screening of dog serum samples Grading of LAT results was done with standard reference serum with known antibody titers A total of 228 serum samples were tested out of which 145 samples were positive by both RFFIT and LAT and the specificity was found to be 100  In RFFIT 151 samples were positive the sensitivity was found to be 9603  and the accuracy/concordance was found to be 9739  A rapid field test—a latex agglutination test LAT—was developed and evaluated for rabies virus antibody assessment using recombinant glycoprotein of rabies virus expressed in an insect cell system

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  16. Isolation of a cytopathogenic virus from a case of porcine reproductive and respiratory syndrome (PRRS) and its characterization as parainfluenza virus type 2
  17. Eukaryotic elongation factor 1-beta interacts with the 5′ untranslated region of the M gene of Nipah virus to promote mRNA translation
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