Authors: Masaaki Kitajima Andri T Rachmadi Brandon C Iker Eiji Haramoto Ian L Pepper Charles P Gerba
Publish Date: 2015/05/05
Volume: 160, Issue: 7, Pages: 1775-1779
Abstract
Human cosavirus HCoSV is a novel member of the family Picornaviridae We investigated the prevalence and genetic diversity of HCoSV in influent and effluent wastewater in Arizona over a 12month period from August 2011 to July 2012 HCoSV sequences were identified in six 25 influent samples and one 4 effluent sample with the highest concentration of 324 × 105 and 154 × 103 copies/liter in influent and effluent respectively The strains were characterized based on their 5’ untranslated region and classified into species A and D demonstrating that genetically heterogeneous HCoSV were circulating with a clear temporal shift of predominant strains in the study areaThe authors would like to thank Kelly Reynolds at the University of Arizona Mel and Enid Zuckerman College of Public Health for her laboratory contributions and two anonymous wastewater treatment plants in southern Arizona for providing wastewater samplesWe also wish to acknowledge the support of the Japan Society for the Promotion of Science JSPS to Masaaki Kitajima under JSPS Postdoctoral Fellowships for Research Abroad FY 2011 no 517 Andri T Rachmadi is a recipient of a 2012 Fulbright Master of Science and Technology Scholarship
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