Authors: R Pantůček J Doškař V Růžičková P Kašpárek E Oráčová V Kvardová S Rosypal
Publish Date: 2004/05/10
Volume: 149, Issue: 9, Pages: 1689-1703
Abstract
Conserved genomic sequences distinctive of Staphylococcus aureus phage types 3A 11 77 187 and Twort representative of phage serogroups A B F L and D were identified and characterized PCR primers designed for the above sequences were used for development of a multiplex PCR assay which enabled us not only to classify all phages of the International Typing Set plus 16 additional phages but also to detect prophages in S aureus genomes One to four different prophages were unambiguously detected in experimentally lysogenized S aureus strains and substantial variation in prophage content was found in 176 S aureus clinical strains of different provenance In addition by using a comparative genomics approach all the prophages in the S aureus genomes sequenced to date could be revealed and classified
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