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Title of Journal: Arch Virol

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Abbravation: Archives of Virology

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Springer Vienna

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DOI

10.1016/0094-730x(91)90008-z

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ISSN

1432-8798

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Eukaryotic elongation factor 1beta interacts with

Authors: Shotaro Uchida Hiroki Sato Misako Yoneda Chieko Kai
Publish Date: 2016/05/28
Volume: 161, Issue: 9, Pages: 2361-2368
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Abstract

Nipah virus belongs to the genus Henipavirus in the family Paramyxoviridae and its RNA genome is larger than those of other paramyxoviruses because it has long untranslated regions UTRs in each gene However the functions of these UTRs are not fully understood In this study we investigated the functions of the 5′ UTRs and found that the 5′ UTR of the M gene upregulated the translation of a reporter gene Using an RNA pulldown assay we showed that eukaryotic elongation factor 1beta EEF1B2 interacts with nucleotides 81–100 of the M 5′ UTR and specifically enhances its translation efficiency Our results suggest that the M 5′ UTR promotes the production of M protein and viral budding by recruiting EEF1B2This work was supported by a GrantinAid for Scientific Research KAKENHI grant number 20790351 and 22228005 from the Japan Society for the Promotion of Science JSPS The massspectrometric analysis was conducted by the Laboratory Center for Proteomics Research Institution of Medical Science The University of Tokyo

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