Authors: Jitendra Kumar Sudhir P Singh Abhinav Kumar Jawaid A Khan Rakesh Tuli
Publish Date: 2012/09/29
Volume: 158, Issue: 1, Pages: 257-261
Abstract
A begomovirus and its associated betasatellites were amplified and sequenced from tobacco plants affected with leaf curl disease The begomovirus was identified as a new strain of tomato leaf curl Pakistan virus ToLCPKV which is referred to here as ToLCPKVIndia A previously known betasatellite tomato leaf curl Patna betasatellite ToLCPaB and a new betasatellite were also found in leafcurlaffected samples The use of infectious clones of ToLCPKVIN plus ToLCPaB for agroinoculation led to typical leaf curl while ToLCPKVIN together with the new betasatellite resulted in curling and chlorosis of leaves Based on these disease symptoms we propose to name the new betasatellite tobacco leaf chlorosis betasatellite TbLChB
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