Authors: Tracy L Meiring Henk Huismans Vida van Staden
Publish Date: 2009/01/20
Volume: 154, Issue: 2, Pages: 263-271
Abstract
The role of African horsesickness virus AHSV nonstructural membrane protein NS3 in determining the effects of AHSV infection on Vero cells was examined NS3 protein sequences are highly variable and cluster into three phylogenetic groups α β and γ Three AHSV strains with NS3 from α β or γ were shown to have quantitatively different phenotypes in Vero cells Reassortants between these strains in which the S10 genome segment encoding NS3 was exchanged alone or with other segments were generated and compared to parental strains Exchange of the NS3 gene resulted in changes in virus release membrane permeability and total virus yield indicating an important role for NS3 in these viral properties Differences in the cytopathicity and the effect on cell viability between the parental strains could not be associated with NS3 alone and it is likely that a number of viral and host factors play a roleThe authors gratefully acknowledge Flip Wege for technical assistance Carel Smit and Eugene Makgopa for their help in generating reassortants and Dr A C Potgieter of the Onderstepoort Veterinary Institute for the cDNA amplification of the viral dsRNA genomes Financial assistance was provided by the National Research Foundation
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