Authors: G Rustici G P Accotto E Noris V Masenga E Luisoni R G Milne
Publish Date: 2014/12/05
Volume: 145, Issue: 9, Pages: 1895-1908
Abstract
An isolate of Indian citrus ringspot virus from Kinnow mandarin in northern India had flexuous particles with evident crossbanding and a modal length of 650 nm It was mechanically transmitted to five herbaceous hosts including Phaseolus vulgaris cv Saxa in which it became systemic In thin sections virus particles were observed in the cytoplasm of parenchyma cells but no specific inclusions were seen The virus was purified from infected Saxa bean leaves and an antiserum prepared There was no serological crossreaction with representative allexi capillo potex and trichoviruses except a faint oneway reaction with Potato virus X Purified virus yielded a major band the presumed coat protein CP of about 34 kDa and a single ssRNA of about 75 kb which was infectious Two ORFs encoding putative proteins of 34 kDa and 23 kDa were located in the 3′ part of the RNA The product of the 34 kDa ORF was confirmed as the CP by expression in E coli The derived amino acid sequence of the CP contained some short motifs similar to those of potex fovea carla and allexiviruses but otherwise there was no strong similarity to any of these The 23 kDa ORF contained a zinc fingerlike sequence as in similar ORFs in carla and allexiviruses but overall amino acid homology with these was low The virus does not appear to fall into any known genus A new species is proposed Serological and molecular diagnostic reagents were prepared
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