Authors: Bing Yuan Hui Fang Chao Shen Congyi Zheng
Publish Date: 2015/06/07
Volume: 160, Issue: 8, Pages: 1989-1999
Abstract
Footandmouth disease virus FMDV is the most contagious pathogen in clovenhoofed twotoed animals Due to the rapid replication and spread of FMDV novel therapeutic strategies are greatly needed to reduce or block FMDV shedding in cases of disease outbreak Here we generated an IRESMx1 construct in which the internal ribosome entry site IRES of FMDV was inserted between the promoter and open reading frame ORF of porcine myxovirus resistance protein 1 poMx1 This construct provides more powerful protection against FMDV infection than the IRESIFN construct that was previously generated by our group The results indicate that this IRESMx1 construct was able to express poMx1 12 h after transfection and induce a robust immune response In contrast to the control the proliferation of virus in transfected cells was significantly inhibited as evaluated by morphology monitoring realtime RTPCR virus titration and Western blot In addition we also found that the antiviral activity in cells transfected with pcIRESMx1 was abolished when the JAK/STAT pathway was repressed which indicates that the antiviral mechanism of poMx1 is JAK/STAT pathway dependent Taken together our data suggest that the antiviral activity of poMx1 is possibly produced by affecting the host cells themselves instead of interacting with the virus directly The new construct reported here could be used as a novel effective therapy against FMDV infectionWe gratefully acknowledge Prof Qingzhen Liu College of Life Science Wuhan University China for critical review of the manuscript We also thank Dr Maoqing Wu Research fellow at Harvard Medical School of Sander International Translation LLC for manuscript polishing service This study was supported by a grant from the National Natural Science Foundation of China No 31370185 and National Infrastructure of Natural Resources for Science and Technology Program No 2011572
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