Authors: E Muller S Sackey
Publish Date: 2004/08/30
Volume: 150, Issue: 1, Pages: 53-66
Abstract
Cacao swollen shoot virus CSSV a member of the family Caulimoviridae genus Badnavirus occurs in all the main cacaogrowing areas of West Africa We amplified cloned and sequenced complete genomes of five new isolates two originating from Togo and three originating from Ghana The genome of these five newly sequenced isolates all contain the five putative open reading frames I II III X and Y described for the first sequenced CSSV isolate Agou1 originating from Togo Their genomes have been aligned with the genome of Agou1 The nucleotide and amino acid sequence identities between isolates have been calculated and a phylogenetic analysis has been made including other pararetroviruses Maximum nucleotide sequence variability between complete genomes of CSSV isolates was 294 Geographical differentiation between isolates appears more important than differentiation between mild and severe isolates ORF X differs greatly in size and sequence between the Togolese isolates Nyongbo2 and Agou1 and the four other isolates its functional role is therefore clearly questionable
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