Authors: Xin Mao Xiaoyun Pan Tao Cheng Xianlong Zhang
Publish Date: 2011/10/01
Volume: 35, Issue: 3, Pages: 905-912
Abstract
Wear particleinduced aseptic loosening has been recognized as a harmful inflammatory process that jeopardizes the longevity of total joint replacement The proteasome controls the activation of NFκB and subsequent inflammatory mediators such as TNFα and IL1β thus we investigated whether proteasome inhibition can ameliorate wear particleinduced inflammation in a murine model A total of 48 BALB/C mice were divided into four groups Titanium Ti particles were injected into the established air pouches of all mice except negative controls to provoke inflammation and then 01 or 05 mg/kg of Bortezomib Bzb a proteasome inhibitor was administered to ameliorate the inflammation response while air pouches without Bzb administration were used as loading controls The air pouches were harvested 2 or 7 days after Bzb injection for molecular and histological analyses Inflammation responses in the air pouch tissues of Bzb treatment groups are lower than those in the Tistimulated group and this occurs in a dosedependent manner Bzb can significantly attenuate the severity of Tiinduced inflammation in air pouches
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