Authors: YuChi Tsung ChihYang Chung HungChieh Wan YaYing Chang PingCheng Shih HanShui Hsu MingChang Kao ChunJen Huang
Publish Date: 2016/12/28
Volume: 40, Issue: 2, Pages: 555-565
Abstract
Inflammation following hemorrhagic shock/resuscitation HS/RES induces acute lung injury ALI Dimethyl sulfoxide DMSO possesses antiinflammatory and antioxidative capacities We sought to clarify whether DMSO could attenuate ALI induced by HS/RES Male SpragueDawley rats were allocated to receive either a sham operation sham plus DMSO HS/RES or HS/RES plus DMSO and these were denoted as the Sham Sham + DMSO HS/RES or HS/RES + DMSO group respectively n = 12 in each group HS/RES was achieved by drawing blood to lower mean arterial pressure 40–45 mmHg for 60 min followed by reinfusion with shed blood/saline mixtures All rats received an intravenous injection of normal saline or DMSO immediately before resuscitation or at matching points relative to the sham groups Arterial blood gas and histological assays including histopathology neutrophil infiltration and lung water content confirmed that HS/RES induced ALI Significant increases in pulmonary expression of tumor necrosis factorα TNFα malondialdehyde nuclear factorkappa B NFκB inducible nitric oxide synthase iNOS and cyclooxygenase 2 COX2 confirmed that HS/RES induced pulmonary inflammation and oxidative stress DMSO significantly attenuated the pulmonary inflammation and ALI induced by HS/RES The mechanisms for this may involve reducing inflammation and oxidative stress through inhibition of pulmonary NFκB TNFα iNOS and COX2 expression
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