Authors: Wei Zhang Zeyou Qi Yaping Wang
Publish Date: 2017/02/07
Volume: 40, Issue: 3, Pages: 778-787
Abstract
Lung ischemiareperfusion I/R injury is a critical complication following a lung transplant cardiopulmonary bypass pulmonary embolism and trauma Immune cells and their effector functions are involved in the lung I/R injury Storeoperated calcium channels SOCC are highly Ca2+selective cation channels and have crucial effects on the immune system It has been indicated that BTP2 a potent SOCC blocker could inhibit proinflammatory cytokine production from immune cells both in vitro and in vivo Therefore this study was conducted to investigate the beneficial effects of BTP2 on lung I/R injury in SpragueDawley SD rats The left lungs of male SD rats underwent ischemia for 60 min and reperfusion for 2 h Treated animals received BTP2 4 mg/kg or 10 mg/kg intraperitoneally 30 min before the ischemia The results revealed that pretreatment with BTP2 markedly attenuated I/R injuryinduced pulmonary edema microvascular protein leakage neutrophil infiltration adhesion molecules cytokine production eg ICAM1 TNFα IL1β and IL2 and the transcription factor nuclear factor of activated T cells c1 nuclear translocation in the lung tissue These findings indicate that BTP2 can be a potential therapeutic drug for lung I/R injury and suggest that SOCC may play a critical role in lung I/R injury
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