Authors: Lin Li Tao Xu Cheng Huang Yunyun Peng Jun Li
Publish Date: 2014/01/21
Volume: 37, Issue: 3, Pages: 835-847
Abstract
The nucleotidebinding domain leucinerich repeat proteins NLRs a class of innate immune receptors that respond to pathogen attack or cellular stress have gained increasing attention NLRC5 is the largest member of NLR family which has recently been identified as a critical regulator of immune responses In this study we explore the role of NLRC5 in cytokine secretion and the role of the JAK2/STAT3 signaling pathway in lipopolysaccharideinduced NLRC5 expression in RAW2647 cells We demonstrated that overexpression of NLRC5 results in a downregulation of interleukin6 IL6 and tumor necrosis factorα TNFα secretion on the other hand knockdown of NLRC5 by transfecting siRNA enhanced IL6 and TNFα secretion in RAW2647 cells These results indicated that NLRC5 plays a negative role in the regulation of IL6 and TNFα Meanwhile AG490 a specific inhibitor of the JAK2/STAT3 signaling pathway and JAK2 siRNA were used to manipulate JAK2/STAT3 activity Finally the results showed that AG490 and JAK2 siRNA inhibited NLRC5 expression and the expression levels of pJAK2 and pSTAT3 We for the first time demonstrate that the inhibition of the JAK2/STAT3 signaling pathway results in decrease of NLRC5 expression
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