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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1002/em.20535

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1573-4978

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The molecular characterization of the brain protei

Authors: Maorong Jiang Xiaosong Gu Xiao Feng Zheng Fan Fei Ding Yan Liu
Publish Date: 2007/11/18
Volume: 36, Issue: 2, Pages: 215-220
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Abstract

The cDNA encoding brain protein 44like Brp44l was identified from the brain and spinal cord cDNA library of Gekko japonicus It contains a 324 bp open reading frame corresponding to a deduced protein of 107 amino acids The molecular weight of the BRP44L protein is 122664 kDa and its theoretical pI is 967 At amino acid level the BRP44L from gecko shares 85–89 similarity with those from other species Northern blotting showed that Brp44l was ubiquitously expressed in the major organs of Gekko japonicus including brain spinal cord heart lung liver and kidney suggesting a fundamental biological role of this gene In situ hybridization ISH revealed that positive hybridization signals were present in gray matter of spinal cord The change of Brp44l expression in spinal cord after tail amputation was examined by reverse transcription polymerase chain reaction RTPCR The expression level of this gene increased in the spinal cord after tail amputation and reached the highest value at 1 week after tail amputation which indicated that the expression level of Brp44l might be associated with the process of spinal cord injury and regenerationThis research was supported by a grant from the National Basic Research Program 973 Program of Ministry of Science and Technology of China 2003CB515306 a grant from the National Natural Science Foundation of China 30600172 and a grant from Basic Research Program of Jiangsu Education Department 05KJA31010

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