Authors: Hadi Mozafari Zohreh Rahimi Azadeh Heidarpour Mahsa Fallahi Adraiana Muniz
Publish Date: 2009/02/15
Volume: 36, Issue: 8, Pages: 2361-2364
Abstract
It has been suggested that the allele frequency of thrombophilic mutations is affected by glucose6phosphate dehydrogenase G6PD deficiency The prevalence of thrombophilic mutations were studied in sixty G6PD deficient individuals including 57 males and three females with the mean age of 15 ± 308 and 110 age and sex matched healthy individuals consisted of 95 males and 15 females with the mean age of 1619 ± 217 from the Kermanshah Province of Iran Using a combination of PCRRFLP technique single strand conformation polymorphism SSCP analysis and DNA sequencing polymorphic G6PD mutations were identified The factor V Leiden prothrombin G20210A and methylenetetrahydrofolate reductase MTHFR C677T were detected by PCRRFLP method using MnlI HindIII and HinfI restriction enzymes respectively Three mutations G6PD Mediterranean G6PD Chatham and G6PD Cosenza were identified in 60 G6PD deficient individuals with highest prevalence of G6PD Mediterranean 916 In G6PD deficient individuals the prevalence of factor V Leiden tended to be higher 5 compared to healthy individuals 27 The prevalence of prothrombin G20210A mutation in G6PD deficient individuals was 17 However in normal subjects the prevalence of this mutation was 27 The frequency of T allele in G6PD deficient individuals were insignificantly higher 2916 than those in healthy individuals 268 Our finding indicates that the prevalence of factor V Leiden prothrombin G20210A and MTHFR C677T in G6PD deficient individuals is not statistically different compared to normal subjects and G6PD deficiency is not associated with these thrombophilic mutations in Western Iran
Keywords: