Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Mol Biol Rep

Search In Journal Title:

Abbravation: Molecular Biology Reports

Search In Journal Abbravation:

Publisher

Springer Netherlands

Search In Publisher:

DOI

10.1002/asna.18380151603

Search In DOI:

ISSN

1573-4978

Search In ISSN:
Search In Title Of Papers:

Overexpression of Emphasis Type="Italic"SpCBL6/

Authors: Yanli Zhou Ying Cheng Yunqiang Yang Xiong Li Basak Supriyo Xudong Sun Yongping Yang
Publish Date: 2016/07/08
Volume: 43, Issue: 9, Pages: 957-966
PDF Link

Abstract

The purpose of the present study was to characterize SpCBL6 GenBank accession number KT780442 from Stipa purpurea and elucidate the function of this protein in abiotic stress The fulllength cDNA of SpCBL6 was isolated from S purpurea by rapid amplification of cDNA ends methods Laser confocal microscopy was used to analyze the subcellular localization of SpCBL6 The constructs of 35SGFPSpCBL6 was used to transform wildtype WT Arabidopsis plants ecotype Columbia0 with the floral dip method Quantitative reversetranscription PCR qRTPCR water potential photosynthetic efficiency F v/F m and ion leakage was performed to investigate the role of SpCBL6 in abiotic stress The open reading frame of SpCBL6 contains 681 bp nucleotides and encodes a 227amino acid polypeptide Phylogenetic analysis indicated that SpCBL6 showed the highest similarity with rice OsCBL6 SpCBL6 transcripts were induced by freezing and drought treatments Subcellular localization analysis showed that SpCBL6 was located in membrane of protoplast Overexpression of SpCBL6 in Arabidopsis thaliana demonstrated that the transgenic plants were more tolerant to cold treatment but less tolerant to drought compared with the plants qRTPCR analysis showed that the drought stress marker genes were inhibited in transgenic plants whereas the cold stress marker genes were enhanced Further analysis showed that SpCBL6overexpressing plants showed enhanced water potential photosynthetic efficiency F v/F m and reduced ion leakage compared with the wildtype after cold treatment Collectively these results indicate that SpCBL6 a new member of the CBL gene family isolated from S purpurea enhances cold tolerance and reduces drought tolerance in plants

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. A novel polymorphisms in intron 12 of the bovine calpastatin gene
  2. Screening of AMP-activated protein kinase α2 subunit interacting proteins by bacterial two-hybrid system
  3. Porcine ubiquitin-like 5 ( UBL5 ) gene: genomic organization, polymorphisms, mRNA cloning, splicing variants and association study
  4. Regulatory mechanism of ZNF139 in multi-drug resistance of gastric cancer cells
  5. Network cluster analysis of protein–protein interaction network identified biomarker for early onset colorectal cancer
  6. Targeting head and neck squamous cell carcinoma using a novel fusion toxin-diphtheria toxin/HN-1
  7. Characterization of nineteen microsatellite markers and development of multiplex PCRs for the wedge clam Donax trunculus (Mollusca: Bivalvia)
  8. Solexa sequencing based transcriptome analysis of Helicoverpa armigera larvae
  9. Association of EphA4 polymorphism with swine reproductive traits and mRNA expression of EphA4 during embryo implantation
  10. Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino
  11. Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters
  12. Modulatory effect of resveratrol on SIRT1, SIRT3, SIRT4, PGC1α and NAMPT gene expression profiles in wild-type adult zebrafish liver
  13. The frequency and distribution of thiopurine S-methyltransferase alleles in south Iranian population
  14. The prevalence and prognostic significance of KRAS mutation in bladder cancer, chronic myeloid leukemia and colorectal cancer
  15. DGGE analysis of buffalo manure eubacteria for hydrogen production: effect of pH, temperature and pretreatments
  16. Effect of mechanical stretch on the expressions of elastin, LOX and Fibulin-5 in rat BMSCs with ligament fibroblasts co-culture
  17. The prevalence of factor V Leiden, prothrombin G20210A and methylenetetrahydrofolate reductase polymorphism C677T among G6PD deficient individuals from Western Iran
  18. Isolation and characterization of the Agvip1 gene and response to abiotic and metal ions stresses in three celery cultivars
  19. XPD Lys 751 Gln and Asp 312 Asn polymorphisms and bladder cancer risk: a meta-analysis
  20. Downregulation of ornithine decarboxylase by pcDNA-ODCr inhibits gastric cancer cell growth in vitro
  21. Cloning, identification and expression analysis of ACC oxidase gene involved in ethylene production pathway
  22. Comparative transcripts profiling of fruit mesocarp and endocarp relevant to secondary metabolism by suppression subtractive hybridization in Azadirachta indica (neem)
  23. The nucleolar protein SURF-6 is essential for viability in mouse NIH/3T3 cells
  24. Transcriptome profiling of the UV-B stress response in the desert shrub Lycium ruthenicum
  25. Lymphatic microvessel density as a prognostic factor in non-small cell lung carcinoma: a meta-analysis of the literature
  26. An alternative genotyping method using dye-labeled universal primer to reduce unspecific amplifications
  27. Molecular cloning of liver Wap65 cDNA in ayu ( Plecoglossus altivelis ) and mRNA expression changes following Listonella anguillarum infection
  28. The glutathione S-transferase M1 and P1 polymorphisms and rheumatoid arthritis: a meta-analysis
  29. Molecular cloning, expression patterns and subcellular localization of porcine TMCO1 gene
  30. A proteomic analysis to identify cold acclimation associated proteins in wild wheat ( Triticum urartu L.)
  31. Molecular cloning, characterization and expression analysis of adenylosuccinate lyase gene in grass carp ( Ctenopharyngodon idella )
  32. NAT2 slow acetylation genotypes contribute to asthma risk among Caucasians: evidence from 946 cases and 1,091 controls
  33. Efficient amplification of light and heavy chain variable regions and construction of a non-immune phage scFv library
  34. Molecular analysis and recombinant expression of bovine neutrophil β-defensin 12 and its antimicrobial activity
  35. The development of 10 novel polymorphic microsatellite markers through next generation sequencing and a preliminary population genetic analysis for the endangered Glenelg spiny crayfish, Euastacus bispinosus
  36. Influence of BLK polymorphisms on the risk of rheumatoid arthritis
  37. Genome wide in silico characterization of Dof gene families of pigeonpea ( Cajanus cajan (L) Millsp.)
  38. Tobacco microRNAs prediction and their expression infected with Cucumber mosaic virus and Potato virus X
  39. Molecular cloning and expression of glycoprotein IIIa T1565C
  40. Gender specificity of a genetic variant of angiotensin-converting enzyme and risk of coronary artery disease
  41. Comprehensive gene and microRNA expression profiling reveals the crucial role of hsa-let-7i and its target genes in colorectal cancer metastasis
  42. Functional characterization of promoter region polymorphisms of human CYP2C19 gene
  43. Genetic polymorphisms and preliminary association analysis with production traits of the porcine SLC27A4 gene
  44. Mutations in lipopolysaccharide-binding protein (LBP) gene change the susceptibility to clinical mastitis in Chinese Holstein
  45. Expression of PiABP19 , Picdc2 and PiSERK3 during induction of somatic embryogenesis in leaflets of Prunus incisa (Thunb.)
  46. Association study of a common genetic variant in pre-miR-1596 with chicken performance traits
  47. HLA association with nasopharyngeal carcinoma in southern Tunisia
  48. A GAL4/UAS luciferase system to identify the miRNAs target mRNAs in Drosophila S2 cells
  49. The molecular characterization of the brain protein 44-like ( Brp44l ) gene of Gekko japonicus and its expression changes in spinal cord after tail amputation
  50. Positive selection pressure within teleost toll-like receptors tlr21 and tlr22 subfamilies and their response to temperature stress and microbial components in zebrafish
  51. The changes of GA level and signaling are involved in the regulation of mesocotyl elongation during blue light mediated de-etiolation in Sorghum bicolor
  52. XPA A23G polymorphism and lung cancer risk: a meta-analysis
  53. RETRACTED ARTICLE: Genetic polymorphisms in VDR , ESR1 and ESR2 genes may contribute to susceptibility to Parkinson’s disease: a meta-analysis
  54. Genetic effects of single nucleotide polymorphisms in JAK2 and STAT5A genes on susceptibility of Chinese Holsteins to mastitis
  55. Molecular characterization of the Salicornia brachiata SbMAPKK gene and its expression by abiotic stress
  56. Proteins involved in biophoton emission and flooding-stress responses in soybean under light and dark conditions
  57. Effects of remifentanyl and fentanyl on LPS-induced cytokine release in human whole blood in vitro
  58. In vivo analysis of translation initiation sites in Plasmodium falciparum
  59. Electroporation is a feasible method to introduce circularized or linearized DNA into B. subtilis chromosome
  60. Comparative modeling and virtual screening for the identification of novel inhibitors for myo-inositol-1-phosphate synthase
  61. Spatiotemporal expression profile of a putative β propeller WDR72 in laying hens
  62. Tissue-specific distribution of uncoupling proteins in normal rats and rats with high-fat-diet-induced obesity
  63. Development of molecular markers specific to petaloid-type cytoplasmic male sterility in tuber mustard ( Brassica juncea var. tumida Tsen et Lee)
  64. Identification of TCP10L as primate-specific gene derived via segmental duplication and homodimerization of TCP10L through the leucine zipper motif
  65. In vitro inhibition of 7-ethoxyresorufin- O -deethylase (EROD) and p -nitrophenol hydroxylase (PNPH) activities by sesamin in hepatic microsomes from two fish species
  66. Association of SCD1 and DGAT1 SNPs with the intramuscular fat traits in Chinese Simmental cattle and their distribution in eight Chinese cattle breeds
  67. Toll-like receptor polymorphisms and vasculitis susceptibility: meta-analysis and systematic review
  68. The mechanism of acidic hydrolysis of esters explains the HDV ribozyme activity
  69. The complete mitochondrial genome of the leafminer Liriomyza trifolii (Diptera: Agromyzidae)
  70. Reprogramming human fibroblasts using HIV-1 TAT recombinant proteins OCT4, SOX2, KLF4 and c-MYC
  71. Molecular cloning, sequence identification, and tissue expression profile analysis of three novel porcine genes: SDHB, SNRPA and CRYBB1
  72. A preliminary in vivo study of the effects of OPN on rat liver regeneration induced by partial hepatectomy
  73. TIM-3 as a new therapeutic target in systemic lupus erythematosus
  74. Helicase: mystery of progression
  75. LIG4 and RAD52 DNA repair genes polymorphisms and systemic lupus erythematosus
  76. Galectin-3 contributes to cisplatin-induced myeloid derived suppressor cells (MDSCs) recruitment in Lewis lung cancer-bearing mice
  77. Screening and identification of natural antisense transcripts in Helicobacter pylori by a novel approach based on RNase I protection assay
  78. Progression of O 6 -methylguanine-DNA methyltransferase and temozolomide resistance in cancer research
  79. Porcine CSRP3 : polymorphism and association analyses with meat quality traits and comparative analyses with CSRP1 and CSRP2
  80. Porcine CSRP3 : polymorphism and association analyses with meat quality traits and comparative analyses with CSRP1 and CSRP2
  81. RNA sequencing reveals differential thermal regulation mechanisms between sexes of Glanville fritillary butterfly in the Tianshan Mountains, China
  82. The polymorphisms of bovine VEGF gene and their associations with growth traits in Chinese cattle
  83. Mexican mestizo population sub-structure: effects on genetic and forensic statistical parameters
  84. Pattern of expression of the CREG gene and CREG protein in the mouse embryo
  85. Matrix Gla protein (MGP) promoter polymorphic variants and its serum level in stenosis of coronary artery
  86. The a1/a2 polymorphism of the glycoprotein IIIa gene and myocardial infarction in Caucasians with type 2 diabetes
  87. Sequence of specific mitochondrial 16S rRNA gene fragment from Egyptian buffalo is used as a pattern for discrimination between river buffaloes, cattle, sheep and goats
  88. Cordyceps sinensis protects against renal ischemia/reperfusion injury in rats
  89. In vitro interaction between the N-terminus of the Ewing’s sarcoma protein and the subunit of RNA polymerase II hsRPB7
  90. Novel identification of Factor XI deficiency in Indian Sahiwal ( Bos indicus ) cattle
  91. Involvement of β-arrestins in cancer progression
  92. Luteolin suppresses growth and migration of human lung cancer cells
  93. Knockdown of the prion gene expression by RNA interference in bovine fibroblast cells
  94. Sequence conservation between porcine and human LRRK2
  95. Should we screen for hereditary hemochromatosis in healthy Lebanese: a pilot study
  96. Levels of hepatitis B virus replicative intermediate in serum samples of chronic hepatitis B patients
  97. Matrine inhibits the growth of retinoblastoma cells (SO-Rb50) by decreasing proliferation and inducing apoptosis in a mitochondrial pathway
  98. Anti- α -actinin antibodies in relation to new-onset systemic lupus erythematosus and lupus nephritis
  99. Hepatocyte growth factor protects endothelial progenitor cell from damage of low-density lipoprotein cholesterol via the PI3K/Akt signaling pathway
  100. Connective Tissue Degeneration: Mechanisms of Palmar Fascia Degeneration (Dupuytren’s Disease)
  101. Variations in the GST activity are associated with single and combinations of GST genotypes in both male and female diabetic patients
  102. Identification and characterization of promoter and regulatory regions for mouse Adam2 gene expression
  103. Intron retention and 3′-UTR analysis of Arabidopsis Dicer-like 2 transcripts
  104. Isolation and characterization of NgRLK1 , a receptor-like kinase of Nicotiana glutinosa that interacts with the elicitin of Phytophthora capsici
Search Result: