Authors: Abu Hena Mostafa Kamal Setsuko Komatsu
Publish Date: 2016/01/11
Volume: 43, Issue: 2, Pages: 73-89
Abstract
To know the molecular systems basically flooding conditions in soybean biophoton emission measurements and proteomic analyses were carried out for floodingstressed roots under light and dark conditions Photon emission was analyzed using a photon counter Gelfree quantitative proteomics were performed to identify significant changes proteins using the nano LC–MS along with SIEVE software Biophoton emissions were significantly increased in both light and dark conditions after flooding stress but gradually decreased with continued flooding exposure compared to the control plants Among the 120 significantly identified proteins in the roots of soybean plants 73 and 19 proteins were decreased and increased in the light condition respectively and 4 and 24 proteins were increased and decreased respectively in the dark condition The proteins were mainly functionally grouped into cell organization protein degradation/synthesis and glycolysis The highly abundant lactate/malate dehydrogenase proteins were decreased in floodingstressed roots exposed to light whereas the lysine ketoglutarate reductase/saccharopine dehydrogenase bifunctional enzyme was increased in both light and dark conditions Notably however specific enzyme assays revealed that the activities of these enzymes and biophoton emission were sharply increased after 3 days of flooding stress This finding suggests that the source of biophoton emission in roots might involve the chemical excitation of electron or proton through enzymatic or nonenzymatic oxidation and reduction reactions Moreover the lysine ketoglutarate reductase/saccharopine dehydrogenase bifunctional enzyme may play important roles in responses in flooding stress of soybean under the light condition and as a contributing factor to biophoton emission
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