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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1007/s10600-011-9916-2

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ISSN

1573-4978

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Identification and characterization of promoter an

Authors: Heejin Choi Boyeon Lee Sora Jin Jun Tae Kwon Jihye Kim Juri Jeong Seungmin Oh ByungNam Cho Zee Yong Park Chunghee Cho
Publish Date: 2012/10/12
Volume: 40, Issue: 2, Pages: 787-796
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Abstract

ADAM2 a member of the ‘a disintegrin and metalloprotease’ ADAM family is a key protein in mammalian fertilization that is specifically expressed in testicular germ cells Here we investigated the transcriptional regulation of the mouse Adam2 gene An in silico analysis identified two conserved noncoding sequences located upstream of the mouse and human ADAM2 genes The upstream region of the mouse Adam2 gene was found to lack typical TATA and CAAT boxes and to have a high GC content Our in vitro transient transfectionreporter analysis identified a promoter in this region of the mouse Adam2 gene along with regulatory regions that inhibit the activity of this promoter in somatic cells Sitedirected mutagenesis revealed that the caudaltype homeobox 1 and CCTCbinding factor motifs are responsible for the inhibitory activities of the repressor regions Finally electrophoretic mobility shift assays showed putative transcription factor–promoter DNA complexes and DNAaffinity chromatography and proteomic analyses identified myelin gene regulatory factor as a binding partner of the Adam2 promoter This provides the first identification and characterization of promoter and repressor regions that regulate the transcription of the mouse Adam2 gene and offers insights into the regulation of this germcellspecific geneThis work was supported by the Korea Science and Engineering Foundation Grant 20100028776 the Korea Research Foundation Grant KRF2008313C00736 and a Gwangju Institute of Science and Technology Systems Biology Infrastructure Establishment Grant

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