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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1002/best.201490002

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ISSN

1573-4978

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Electroporation is a feasible method to introduce

Authors: MingMing Yang WeiWei Zhang XiaoTing Bai HengXin Li PeiLin Cen
Publish Date: 2009/08/11
Volume: 37, Issue: 5, Pages: 2207-2213
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Abstract

Here we present the electroporation as a feasible and efficient method for introducing circularized and linearized DNA into Bacillus subtilis chromosome Two integration experiments were carried out and demonstrated the feasibility and efficiency of electroporation to introduce the target DNA into the B subtilis chromosome By using of electroporation a multiplecistron contained five genes from B subtilis biotin biosynthetic pathway was introduced into the B subtilis chromosome efficiently and created a repeated copy in chromosome via a single crossover event Then an ectopic promoter was introduced conveniently into the upstream of one of the repeated multiplecistron via a double crossover event To further demonstrate the application of electroporation in genetic research the early sporulation gene spo0A of B subtilis was knocked out and consequently the null of sporulation and logged growth was observed in this study Thus the electroporation as an alternative method of integration in B subtilis is feasible and practical

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