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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1002/macp.1971.021500114

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ISSN

1573-4978

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Identification of Emphasis Type="Italic"TCP10L/

Authors: Zhaomin Zhong Jianping Qiu Xinya Chen Bo Wan Jun Ni Yun Yang Meirong Bai Haoxing Zhang Long Yu
Publish Date: 2007/03/22
Volume: 35, Issue: 2, Pages: 171-178
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Abstract

TCP10L a transcriptional repression factor gene that was localized on human chromosome 21q2211 was identified to be derived through segmental duplication since the divergence of primates and rodents It was elucidated that TCP10L gene was a primatespecific gene in this study Subsequently it was demonstrated that the putative leucine zipper motif mediated the homodimerization of TCP10L Using in vitro and in vivo methodologies it was shown that either deletion or point mutation of the leucine zipper motif was sufficient to abolish TCP10L homodimerization In Hela cells both the exogenous wild type TCP10L and endogenous TCP10L were detected on nuclei with immunofluorescence assay However the leucine zipper motif mutants of TCP10L could also be detected on nuclei The results suggested that the leucine zipper motif enabled TCP10L to homodimerize but was not essential for the TCP10L nuclear localization

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