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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1016/0375-9474(91)90702-8

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ISSN

1573-4978

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Screening of AMPactivated protein kinase α2 subun

Authors: QingYing Fu YuQi Gao
Publish Date: 2007/11/22
Volume: 36, Issue: 2, Pages: 337-344
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Abstract

AMPactivated protein kinase AMPK is a ubiquitous eukaryotic protein kinase regulating cellular metabolism and energy demand In brain AMPK plays a role as a multidimensional energy sensor and AMPK α2 subunit is expressed at higher levels than AMPK α1 subunit In order to identify potential targets of AMPK in brain we perform bacterial twohybrid screening of a rat fetal brain cDNA library using AMPK α2 subunit as bait Here we present seven potential AMPK α2 subunit interacting proteins including 6phosphofructo1kinase PFK1 polyubiquitin cytochrome c oxidase subunit I COX I heat shock protein 8 HSP8 HLABassociated transcript 3 BAT3 isoform 1 protein tyrosine phosphatase receptor type D PTPRD and isletbrain 1 IB1 They are involved in glycolysis protein degradation mitochondrial electron transport and apoptosis pathways participating in energy regulation directly or indirectly These data may provide new insight into further studying the pathways of AMPK energy regulation in brain and possible mechanisms of AMPKmediated neuroprotective effect

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