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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1007/bf03160689

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ISSN

1573-4978

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A preliminary in vivo study of the effects of OPN

Authors: Gaiping Wang Congcong Zhao Shasha Chen Xiaofang Li Ling Zhang Cuifang Chang Cunshuan Xu
Publish Date: 2016/09/01
Volume: 43, Issue: 12, Pages: 1371-1382
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Abstract

Osteopontin OPN is a member of Th1 cytokine secreted by activated lymphocytes and macrophages However it deserves to be studied whether OPN could promote cell activation or proliferation and then facilitate hepatic selfrepair during liver regeneration LR This study is designed to further reveal the effects of OPN on LR in vivo Firstly quantitative reverse transcriptionPCR qRTPCR and western blot WB were utilized to validate the expression profile of endogenous OPN in rat regenerating livers after partial hepatectomy PH Then OPN expression vector two shRNA expression vectors and their respective test vectors were successfully constructed Afterwards test vectors were administrated into mouse livers via tail vein to find the more efficient shRNA Furthermore OPN expression vector and the more efficient shRNA expression vector were injected into rat regenerating livers and then the changes in liver regeneration and hepatic microstructure were respectively detected by liver regeneration rate and HE staining while the expressions of several marker genes were detected by qRTPCR and WB Endogenous OPN was strikingly upregulated in both mRNA and protein level during LR especially at 12 and 72 h after PH The shRNA expression vector Opn313 was found to be more efficient than Opn887 in silencing the expression of Opn Then OPN expression vector and Opn313 were injected into rat remnant livers and it showed that OPN overexpression aggravated hepatic necrosis and leukocytes infiltration while OPN silencing inhibited liver regeneration rate and the expressions of PCNA and CCL2 but augmented that of BAX In conclusion OPN might enhance inflammation and cell proliferation attenuate cell apoptosis and ultimately facilitate liver regeneration at the termination stage of liver regenerationSupported by National Natural Science Foundation Project of China Grant No 81200317 Natural Science Foundation of Henan Province Grant No 142300413227 Key Scientific Research Projects of Henan Province Grant No 15A180007 and Doctoral Scientific Research Startup Foundation of Henan Normal University Grant No 11128

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