Authors: Zohreh Jafari Raheem Haddad Ramin Hosseini Ghasemali Garoosi
Publish Date: 2012/10/18
Volume: 40, Issue: 2, Pages: 1341-1350
Abstract
1aminocyclopropane1carboxylic acid oxidase ACO enzyme is a member of the Fe IIdependent family of oxidases/oxygenases which require Fe2+ as a cofactor ascorbate as a cosubstrate and CO2 as an activator This enzyme catalyses the terminal step in the plant signaling of ethylene biosynthetic pathway A 948 bp fragment of the ACO1 gene cDNA sequence was cloned from tomato Lycopersicon esculentum fruit tissues by using reverse transcriptasepolymerase chain reaction RTPCR with two PCR primers designed according to the sequence of a tomato cDNA clone X58273 The BLAST search showed a high level of similarity 77–98 between ACO1 and ACO genes of other plants The calculated molecular mass and predicted isoelectric point of LeACO1 were 358 kDa and 513 respectively The threedimensional structure studies illustrated that the LeACO1 protein folds into a compact jellyroll motif comprised of 8 αhelices 12 βstrands and several long loops The cosubstrate was located in a cofactorbinding pocket referred to as a 2His1carboxylate facial triad Semiquantitative RTPCR analysis of gene expression revealed that the LeACO1 was expressed in fruit tissues at different ripening stages
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