Authors: Martin Masopust Filip Weisz Heinz Bartenschlager Aleš Knoll Zuzana Vykoukalová Hermann Geldermann Stanislav Čepica
Publish Date: 2014/01/24
Volume: 41, Issue: 4, Pages: 2353-2362
Abstract
Ubiquitinlike 5 UBL5 which is supposed to be involved in regulation of feed intake energy metabolism obesity and type 2 diabetes is located at position 621 cM on the pig chromosome 2 region harbouring quantitative trait loci for carcass and meat quality The 4354 bp genomic sequence FR798948 of the porcine gene encompassing the promoter and entire gene was cloned by polymerase chain reaction Comparative sequencing revealed 13 polymorphisms in noncoding regions Synthesis of fulllength cDNA sequences using rapid amplification of 5′ and 3′ ends showed three splice variants Variants 1 and 2 differ in transcription length for the untranslated part of exon 1 with deduced protein of 73 amino acid aa residues and 100 identities between human mouse and other species Variant 3 with 4 bp deletion at the 3′ end of exon 2 encodes a truncated protein with 28 aa residues In a Wild boar×Meishan F2 population n = 334 with 47 recorded traits loci FR798948g2788GA and FR798948g2141TC were associated at nominal P 005 with fat deposition growth and fattening and muscling but after adjustment for multiple testing Benjamini and Hochberg J R Stat Soc B 57289–300 1995 only eight fat deposition traits showed suggestive association with FR798948g2788GA at adjusted P 010 In a Meishan×Large White MLW cross n = 562 with six trait records available FR798948g2141TC showed suggestive association with growth adjusted P = 00690 As association mapping conducted in the outbred MLW population is more precise than in the three generation F2 population the UBL5 gene tends to be associated with growth rather than with fat accretionThe authors thank Petra Šejnohová for technical assistance DNA samples and trait records of the MLW cross were kindly provided by Genus plc De Forest Wisconsin USA This work was supported by the Czech Science Foundation Grant No P502/10/1216 Institute of Animal Physiology and Genetics AS CR vvi RVO 67985904 CEITEC project CZ105/1100/020068
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