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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1016/0022-3093(93)90322-o

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ISSN

1573-4978

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Downregulation of ornithine decarboxylase by pcDNA

Authors: Chunxiao Xu Yunfei Yan Yapei Yang Bin Liu Jiaxuan Xin Shimin Chen Wei Wang Chunying Jiang Zhaoxin Lu Xianxi Liu
Publish Date: 2010/06/09
Volume: 38, Issue: 2, Pages: 949-955
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Abstract

Ornithine decarboxylase ODC the first ratelimiting enzyme of polyamine biosynthesis was found to be associated with cell growth proliferation and transformation ODC gene expression in gastric cancer was increased and its level was positively correlated with the degree of malignity of gastric mucosa and development of gastric lesions In order to evaluate the therapeutic effects of antisense RNA of ODC on gastric cancer an antisense RNA of ODC expressing plasmid pcDNAODCr which delivered a 120 bp fragment complementary to the initiation codon of ODC gene was constructed and transfected to gastric cancer cells SGC7901 and MGC803 Expression of ODC in gastric cancer cells was determined by western blot Cell proliferation was assessed by MTS assay Cell cycle was analyzed by flow cytometry and Matrigel assay was performed to assess the ability of gastric cancer cell invasiveness The results showed that the ODC gene expression in gastric cancer cells transfected with the pcDNAODCr was downregulated efficiently Tumor cell proliferation was suppressed significantly and cell cycle was arrested at G1 phase Gastric cancer cells had reduced invasiveness after gene transfer Our study suggested that antisense RNA of ODC expressing plasmid pcDNAODCr had antitumor activity by inhibiting the expression of ODC and downregulation of ODC expression using a gene therapy approach might be a novel therapeutic strategy for gastric cancer

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