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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1016/0304-4238(83)90108-5

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ISSN

1573-4978

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Molecular cloning sequence identification and ti

Authors: Q C An G Y Liu
Publish Date: 2008/03/22
Volume: 36, Issue: 4, Pages: 683-690
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Abstract

The complete coding sequences of three porcine genesSDHB SNRPA and CRYBB1 were amplified using the reverse transcriptase polymerase chain reaction RTPCR based on the conserved coding sequence information of the mouse or other mammals and highly homologous pig ESTs These three genes were then deposited into GenBank database and assigned to GeneID 100125544 768109 and 780429 The phylogenetic tree analysis revealed that the swine SDHB and SNRPA have closer genetic relationships with the human SDHB and SNRPA but swine CRYBB1 has a closer genetic relationship with the bovine CRYBB1The tissue expression analysis indicated that that swine SDHB SNRPA and CRYBB1 gene were differentially expressed in tissues including fat lung muscle small intestine kidney large intestine spleen and liver Our experiment is the first to establish the primary foundation for further research on these three swine genes

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