Authors: Javad Gharechahi Houshang Alizadeh Mohammad Reza Naghavi Golandam Sharifi
Publish Date: 2014/02/18
Volume: 41, Issue: 6, Pages: 3897-3905
Abstract
To gain a better understanding of cold acclimation process in wheat we applied a 2DE based proteomic approach to discover changes in proteome profile of a diploid wild wheat Triticum urartu L during prolonged cold stress treatment To this end plants were grown in pots and the growing seedlings 4leaf stage were exposed to cold stress After 4 weeks of cold acclimation 4–6 °C and subsequent treatment for 12 h at −2 °C samples were collected from control and stressed plants and were subjected to proteome pattern analysis Among approximately 450 reproducible protein spots displayed in each given 2DE gels 34 proteins changed significantly in abundance in response to cold stress Among them 25 and 9 proteins were up and downregulated under stress condition respectively Analysis by matrixassisted laser desorption ionization time of flight/time of flight mass spectrometry coupled with nonredundant protein database search allowed the identification of 20 coldinduced proteins Integrated proteomic and database survey resulted in identification of several cold stress related proteins such as pathogenesis related protein cold regulated protein coldresponsive LEA/RABrelated COR protein oxygenevolving enhancer protein and oxalate oxidase The presumed functions of the identified proteins were mostly related to cold acclimation oxidative stress and photosynthesis The possible implications of differentially accumulated proteins in acquiring systemic tolerance to freezing stress following exposure to prolonged low temperature will be discussed
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