Authors: Wenwen Lv Jie Ding Jiangbo Hu Chao Wang Leyi Xu Ming Fang Liudi Yuan
Publish Date: 2008/09/23
Volume: 36, Issue: 6, Pages: 1627-1631
Abstract
MicroRNAs miRNAs have been shown to regulate gene expression through the sequencespecific base pairing with their target mRNAs However our understanding of the biological roles of miRNAs is still quite limited and only a handful of miRNAs have been assigned by genetic analysis in part owing to the difficulty in the identification of their targets Although computational methods have shown to be helpful in the prediction of miRNA targets a major obstacle has been the lack of quick and efficient experimental procedures to verify these targets In this report we describe a UAS/GAL4based reporter system for this purpose Our data indicate it an assay of miRNA–target gene interaction with greater sensitivity over the previously reported methods and may be useful for more efficient identification/validation the miRNA targets in Drosophila cell lines
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