Authors: Guitang Yang Yaling Han Xiaoxiang Tian Jie Tao Mingyu Sun Jian Kang Chenghui Yan
Publish Date: 2010/09/21
Volume: 38, Issue: 3, Pages: 2133-2140
Abstract
The cellular repressor of E1Astimulated genes CREG is a secreted glycoprotein that inhibits cell proliferation and/or enhances differentiation CREG is widely expressed in adult tissues such as the brain heart lungs liver intestines and kidneys in mice We investigated the level of CREG expression during mouse embryogenesis and its distribution at 185 days post coitus dpc using immunohistochemical staining with diaminobenzidine western blotting and reverse transcriptionpolymerase chain reaction CREG expression was first detected in mouse embryos at 45 dpc It was expressed at almost all stages up to 185 dpc The level of CREG was found to increase gradually and was highest at 185 dpc Western blotting showed that the CREG protein was expressed at higher levels in the brain heart intestines and kidneys than in the lungs and liver at 185 dpc In 95 dpc embryos CREG was expressed only in the endothelial cells of blood vessels after the vascular lumen had formed With advanced differentiation vascular smooth muscle cells developed in the embryonic vascular structures the expression of smooth muscle αactin protein and CREG were positive and increased gradually in 105 dpc embryonic vessels CREG expression in the embryonic blood vessels peaked at 155 dpc and was reduced slightly at 185 dpc These results indicate that CREG is expressed during mouse embryogenesis and might participate in the differentiation of these organs during embryogenesis
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