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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1016/0005-2760(82)90270-3

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1573-4978

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Reprogramming human fibroblasts using HIV1 TAT re

Authors: Chuanying Pan Baisong Lu Hong Chen Colin E Bishop
Publish Date: 2009/08/09
Volume: 37, Issue: 4, Pages: 2117-2124
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Abstract

It has been shown that human and murine fibroblasts can be reprogrammed by ectopic expression of transcription factors using viral vectors For the purpose of human therapeutic applications the integration of viral transgenes into the genome is unlikely to be accepted We therefore produced recombinant transcription factor proteins in E coli OCT4 SOX2 cMYC and KLF4 carrying the cell penetrating TAT domain from HIV1 The purified proteins were able to enter into mammalian cells when added to tissue culture medium but appeared not to translocate to the nucleus Further investigation indicated that most of the protein was tied up in the endosomes and was unavailable for reprogramming Once this problem has been solved it seems likely that protein reprogramming will be the method of choice for clinical applicationsWe are grateful to Lisa Dailey for providing the 6X0/Sluc luciferase reporter plasmid We thank Zhan Wang Wake Forest Institute for Regenerative Medicine for his help in experiments C Pan was supported by CHINA SCHOLARSHIP COUNCIL This work was supported by a grant from National Institutes of Health NIH R21 RR025408

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