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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1007/bf00123871

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ISSN

1573-4978

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Molecular characterization of the Emphasis Type="

Authors: Pradeep K Agarwal Kapil Gupta Bhavanath Jha
Publish Date: 2009/08/28
Volume: 37, Issue: 2, Pages: 981-
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Abstract

MAPK cascade is an important intracellular signaling module and function as a convergent point for crosstalk during abiotic stress signaling In this study SbMAPKK gene has been isolated from Salicornia brachiata a highly salt tolerant plant growing in costal marshes of Gujarat India The SbMAPKK gene is 1023 bp long encodes a 340 amino acid protein with an estimated molecular mass of 374 kDa The SbMAPKK shows high sequence identity with NbMKK1 from N benthamiana LeMKK4 from Lycopersicon esculentum SbMAPKK constitutes 11 conserved subdomains of protein kinase Northern analysis revealed that SbMAPKK transcript expression is induced by different stresses like dehydration cold and salt however maximum expression is observed during cold stress The phylogenetic analysis and genomic organization confirms that it is an intron less gene belonging ‘D’ group in MAPKK family

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