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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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1573-4978

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Characterization of nineteen microsatellite marker

Authors: Ana Nantón Alberto AriasPérez Josefina Méndez Ruth Freire
Publish Date: 2014/05/23
Volume: 41, Issue: 8, Pages: 5351-5357
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Abstract

The wedge clam Donax trunculus is an AtlanticMediterranean warmtemperate species found from Senegal to the northern coast of France including the Mediterranean and Black Sea It is commercially exploited in several European countries and constitutes an important fishing resource due to its high economical value To contribute to its conservation and management nineteen microsatellite markers were isolated from two enriched genomic libraries These loci were characterized in 30 clams from a single population from northwest Spain The number of alleles per locus ranged from 2 to 17 and observed and expected heterozygosity varied from zero to 0714 and from 0078 to 0950 respectively Linkage disequilibrium was not detected and nine loci were in agreement with Hardy–Weinberg equilibrium Fifteen polymorphic markers were arranged into three multiplex PCR sets to reduce both time and cost of microsatellite genotyping This is the first time that polymorphic microsatellite markers have been reported for D trunculus These new markers provide a valuable resource for future population genetics studies and management and culture of this speciesWe thank Dra Dorotea Martínez and Susana Nóvoa from Centro de Cultivos Marinos de Ribadeo—CIMA Xunta de Galicia for supplying the wedge clam samples Jose García Gil for his technical assistance and Luisa Martínez for helpful comments on earlier versions of this manuscript We would also like to thank Dr James D Austin for their revision of English grammar and style This work was supported by the Consellería de Economía e Industria Xunta de Galicia through project 10MMA103013 and by Ministerio de Educación through a predoctoral FPU fellowship awarded to Ana Nantón

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