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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1016/0003-9861(56)90222-3

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ISSN

1573-4978

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Expression of Emphasis Type="Italic"PiABP19/Emp

Authors: Kaouther Ben Mahmoud Fabienne Delporte Yordan Muhovski Nadhra Elloumi Ahmed Jemmali Philippe Druart
Publish Date: 2012/10/21
Volume: 40, Issue: 2, Pages: 1569-1577
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Abstract

Somatic embryogenesis is a useful tool of plant breeding In this context a procedure for inducing somatic embryogenesis in Prunus incisa leaf explants had been previously developed The original in vitro protocol relies on picloram treatments and exposure to darkness as inductive conditions the best frequency of embryogenesis being obtained on the second leaf F2 exposed to 4 μM picloram during 30 days The morphological and biochemical changes observed during somatic embryogenesis occur in response to alterations in gene expression regulation patterns A molecular study was conducted in order to provide deeper insight into the fundamental biological factors involved in the induction of this process using a gene candidate strategy and semiquantitative reverse transcription polymerase chain reaction analysis So far no sequence data related to somatic embryogenesis has been available in cherry In the present study we cloned and sequenced cDNA fragments of putative genes encoding auxinbinding protein cell cycle regulator and somatic embryogenesis receptor kinase Timecourse differential transcript accumulations were observed for all investigated genes in leaves or derived callus tissues during the observation period first month of culture Their possible involvement in the sequential steps of the embryogenic pathway dedifferentiation cell proliferation differentiation through somatic embryogenesis is presented and discussed

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