Authors: Z Y Xu Y Z Xiong M G Lei F E Li B Zuo
Publish Date: 2008/08/12
Volume: 36, Issue: 6, Pages: 1427-1432
Abstract
Solute carrier family 27 fatty acid transporter member 4 SLC27A4 is a fatty acylCoA synthetase producing very long chain fatty acidCoA for lipid metabolic pathways suggesting that the SLC27A4 gene is a potential candidate gene for traits related to fat deposition in animals This study was conducted to sequence the genomic region from exon 6 to 12 of porcine SLC27A4 and detect polymorphisms by comparative sequencing In silico mapping assigned SLC27A4 gene between gene COQ4 coenzyme Q4 homolog and URM1 ubiquitin related modifier 1 homolog on pig chromosome 1q24q212 where significant QTL affecting backfat depth had previously been identified Thirty six putative sites of variation were detected of which 31 polymorphisms including 28 SNPs and 3 indels were located in the intronic region and 5 in the exonic regions The g1777GA EU703769 in intron 8 was confirmed by PCRRFLP using HpaII restriction enzyme and further genotyped in four Chinese native pig breeds Meishan Erhualian Tongcheng and Qingping and three western meattype pig breeds Duroc Large White and Landrace Allele G was exclusively present in Tongcheng and Qingping pigs and predominant in the other pig populations analyzed Significant differences of backfat at rump body weight at birth and average daily gain on weaning between the AG and GG genotype were observed in Landrace pig population P 005This study was supported financially by National Natural Science Foundation of PR China 30500358 the National High Technology Development Project 2007AA10Z162 2006AA10Z1D6 and the National “973” Program of PR China 2006CB102102 The authors also acknowledge the teachers and graduate students of the Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture and the Swine Breeding Center of China for the traits collection
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