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Title of Journal: Mol Biol Rep

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Abbravation: Molecular Biology Reports

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Springer Netherlands

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DOI

10.1007/s10243-007-0112-9

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ISSN

1573-4978

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Sequence of specific mitochondrial 16S rRNA gene f

Authors: Hassan A I Ramadan
Publish Date: 2010/11/30
Volume: 38, Issue: 6, Pages: 3929-3934
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Abstract

Characterization of molecular markers and the development of better assays for precise and rapid detection of domestic species are always in demand This is particularly due to recent food scares and the crisis of biodiversity resulting from the huge ongoing illegal traffic of endangered species The aim of this study was to develop a new and easy method for domestic species identification river buffalo cattle sheep and goat based on the analysis of a specific mitochondrial nucleotide sequence For this reason a specific fragment of Egyptian buffalo mitochondrial 16S rRNA gene 422 bp was amplified by PCR using two universal primers The sequence of this specific fragment is completely conserved between all tested Egyptian buffaloes and other river buffaloes in different places in the world Also the lengths of the homologous fragments were less by one nucleotide 421 bp in case of goats and two nucleotides 420 bp in case of both cattle and sheep The detection of specific variable sites between investigated species within this fragment was sufficient to identify the biological origin of the samples This was achieved by alignment between the unknown homologous sequence and the reference sequences deposited in GenBank database accession numbers FJ748599–FJ748607 Considering multiple alignment results between 16S rRNA homologous sequences obtained from GenBank database with the reference sequence it was shown that definite nucleotides are specific for each of the four studied species of the family Bovidae In addition other nucleotides are detected which can allow discrimination between two groups of animals belonging to two subfamilies of family Bovidae Group one closely related species like cattle and buffalo Subfamily Bovinae and Group two closely related species like sheep and goat Subfamily Caprinae This 16S DNA barcode characterbased approach could be used to complement cytochrome c oxidase I COI in DNA barcoding Also it is a good tool for identification of unknown sample belonging to one of the four domestic animal species of family Bovidae quickly and easily

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