Authors: Mohammed A Alhomidi Bhavani Vedicherla Sireesha Movva Prabhakar K Rao Yog R Ahuja Qurratulain Hasan
Publish Date: 2013/05/03
Volume: 34, Issue: 4, Pages: 2427-2432
Abstract
Somatic mutations in mitochondrial DNA mtDNA have been demonstrated in various tumors Mitochondrial Dloop is a noncoding region in the mitochondrial genome which has essential transcription and replication elements and alterations in this region may affect both these processes The Dloop has a polyC tract PCT located between 303 and 315 nucleotides known as D310 which has been identified as a frequent hot spot mutation region in human neoplasia In the present study 77 pairs of breast tumor and adjacent nontumorous tissue samples were analyzed by polymerase chain reaction–singlestrand conformational polymorphism restriction fragment length polymorphism and sequencing to evaluate the frequency of D310 PCT mutations and its association with clinicopathologic parameters of breast cancer Alterations were detected in 25 of 77 325 breast cancer samples these included 7/25 28 cases with heteroplasmy This is the first study from Asian Indian breast cancer BC patients indicating a relatively high frequency of D310 mutations suggesting that mtDNA instability at D310 may be a common characteristic of BC However 667 of the alterations were observed in stage II BC indicating that this may be a more important change for early progression of the disease rather than its initiationThis work was supported by the Ministry of Health Yemen and the Department of Science and Technology DST India grant no SR/SO/HS89/2004 The authors would like to thank the members of the Department of Pathology Kamineni Hospital for providing BC tissue samples
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