Authors: Chuifeng Fan Yang Zhao Xiaoyun Mao Yuan Miao Xuyong Lin Guiyang Jiang Xiupeng Zhang Qiang Han Lan Luan Enhua Wang
Publish Date: 2014/08/14
Volume: 35, Issue: 11, Pages: 11337-11343
Abstract
Armadillo repeatcontaining protein 8 Armc8 is a key factor to regulate cell membrane adhesion complex through promoting αcatenin degradation However its expression and function in human malignant tumors are largely unknown Here we present our study investigating Armc8 expression in tumor and nontumor breast tissues including 45 normal epithelia 53 lesions of hyperplasia with or without dysplasia 22 benign tumors and 92 carcinomas including 28 carcinomas in situ and 64 infiltrating carcinomas using immunohistochemistry IHC and Western blotting study Armc8 expression was detected mainly in the cytoplasm with occasional membrane immunostaining The positive rate of Armc8 expression in normal breast epithelia 89 four out of 45 was very low No significant difference was found between Armc8 expression in usual ductal hyperplasia UDH 111 two out of 18 benign breast tumors including intraductal papilloma 100 one out of 10 and fibroadenoma 83 one out of 12 and normal breast epithelia p 005 Elevated expression of Armc8 was found in breast epithelia with dysplasia 240 six out of 25 compared to that in normal breast epithelia UDH and benign breast tumors p 005 Armc8 expression in breast carcinoma including breast carcinoma in situ 10/28 357 infiltrating ductal carcinoma 607 34/56 and infiltrating lobular carcinoma 500 4/8 was higher than that in normal breast epithelia UDH benign breast tumors and breast epithelia with dysplasia p 005 The highest expression of Armc8 was found in infiltrating breast carcinoma 594 38/64 compared to all the other breast tissues Higher Armc8 expression was found to be linked to lymph node metastasis and advanced tumor–node–metastasis TNM stages III + IV in infiltrating breast carcinoma p 005 We further confirmed Armc8 expression in breast epithelial cell line MCF10A and breast carcinoma cell lines including MCF7 MDAMB231 and ZR751 using Western blotting and immunofluorescent study These results indicate that the elevated expression of Armc8 may be involved in carcinogenesis including atypiatocarcinoma progression and cancer development of breast carcinoma
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