Authors: Sen Guo Hongda Liu Yanfeng Liu Lei Liu Qiang Sun Xijun Cui
Publish Date: 2014/09/28
Volume: 36, Issue: 1, Pages: 353-364
Abstract
Hepatomaderived growth factor HDGF is an acidic heparinbinding protein involved in tumor progression and poor prognosis of kinds of cancers Aimed at investigating the functions of HDGF in intrahepatic cholangiocarcinoma IHCC we detected the expression of HDGF by immunohistochemistry in 83 patients Associations of HDGF with clinicopathologic features microvascular density MVD and overall survival rates were further analyzed by Chisquare method univariate or multivariate analysis HDGF functions in IHCC proliferation invasion and angiogenesis were detected by MTT transwell and tube formation assays respectively As a result we found that HDGFpositive expression rate in IHCC was 518 43/83 in IHCC HDGF expression was significantly correlated to MVD P = 0031 lymphatic invasion P = 0030 distant metastasis P = 0002 and TNM stage P = 0037 HDGF was further identified as an independent prognostic factor in IHCC with KaplanMeier method P = 0003 and Coxregression model P = 0008 Moreover both intracellular and extracellular HDGF were proved to promote the proliferation invasion and angiogenesis of IHCC cell lines In conclusion HDGF was identified as an independent prognostic biomarker in IHCC HDGF can promote IHCC cells progression including proliferation invasion and angiogenesis indicating HDGF could become a new promising and potential drug target of IHCC
Keywords: