Authors: Xiabin Lan Wei Sun Ping Zhang Liang He Wenwu Dong Zhihong Wang Siming Liu Hao Zhang
Publish Date: 2015/11/26
Volume: 37, Issue: 5, Pages: 6117-6123
Abstract
Long noncoding RNA lncRNA is a kind of RNA that is longer than 200 nucleotides with limited or no proteincoding potential Studies have proved that lncRNAs play important regulatory roles in gene expression and contribute to oncogenesis and cancer metastasis However the expression level of lncRNAs and their clinicopathologic significance in papillary thyroid carcinoma PTC have not been well studied In this study we investigated the expression level of a novel lncRNA NONHSAT037832 in PTC and paired noncancerous thyroid tissues as well as some cell lines by quantitative realtime polymerase chain reaction The association between the expression level of NONHSAT037832 and clinicopathologic characteristics of patients with PTC was further analyzed Three receiver operating characteristic curves ROCs were established to evaluate the diagnostic value of NONHSAT037832 The results suggested that the expression level of NONHSAT037832 was significantly decreased in PTC compared with paired noncancerous tissues P 001 And NONHSAT037832 was also significantly downregulated in two PTC cell lines K1 and IHH4 compared to normal thyroid follicular epithelial cell line Nthyori 31 P 001 Downregulated NONHSAT037832 was significantly associated with lymph node metastasis P = 0015 and tumor size P = 0032 The ROCs revealed that NONHSAT037832 had a high diagnostic value for differentiating between PTC and noncancerous diseases as well as identifying PTC with lymph node metastasis and larger tumors ≥3 cm The area under curve was up to 0897 95CI = 0852–0942 P = 0000 0641 95CI = 0519–0762 P = 0033 and 0702 95CI = 0567–0827 P = 0008 respectively This study indicated that NONHSAT037832 might serve as a potential biomarker of PTC
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