Authors: Kejia Wang Qing Liang Li Wei Wei Zhang Ping Zhu
Publish Date: 2015/10/16
Volume: 37, Issue: 3, Pages: 3823-3830
Abstract
Human hepatocellular carcinoma HCC is one of the most prevalent malignancies in the world Research on HCC has recently focused on microRNAs miRNAs that play crucial roles in cancer development and progression of HCC In this study we aimed to analyze the expression and function of a metastasisassociated microRNA608 miR608 in HCC Samples of human HCC and matched adjacent normal tissues were surgically removed and miR608 expression and the pathological characteristics of HCC were investigated In this study we found that miR608 expression was significantly reduced in HCC and its expression levels were highly associated with tumor size differentiation clinical stage and overall and diseasefree survival of HCC Overexpression of miR608 in HCC cell lines HepG2 and SKHep1 inhibited cell proliferation by G1 arrest Macrophage migration inhibitory factor MIF a potential target gene of miR608 was inversely correlated with miR608 expression in HCC tissues and cell lines Furthermore we demonstrated that MIF was directly regulated by miR608 and the restoration of MIF expression reversed the inhibitory effects of miR608 on HCC cell proliferation Taken together these findings collectively demonstrate that miR608 exerts its anticancer function by directly targeting MIF in HCC indicating a potential novel prognostic biomarker and therapeutic target for HCC
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