Authors: Xiaojun Man Jiani He Chuize Kong Yuyan Zhu Zhe Zhang
Publish Date: 2014/01/18
Volume: 35, Issue: 5, Pages: 4131-4136
Abstract
Caspase recruitment domain and membraneassociated guanylate kinaselike domain protein 3 CARMA3 was reported as an oncoprotein overexpressed in several cancers The expression pattern of CARMA3 and its clinical significance in human bladder cancer have not been well characterized In the present study CARMA3 expression was analyzed in 90 archived bladder cancer specimens using immunohistochemistry and the correlation between CARMA3 expression and clinicopathological parameters was evaluated We found that CARMA3 was overexpressed in 35 of 90 388 bladder cancer specimens Significant association was observed between CARMA3 overexpression with tumor status p = 0081 and tumor grade p = 0027 To further explore the biological functions of CARMA3 in bladder cancer we depleted CARMA3 in T24 and 5637 cell lines using small interfering RNA siRNA Using cell counting kit8 CCK8 assay and colony formation assay we were able to show that CARMA3 depletion inhibited cell proliferation and colony number Further study demonstrated that CARMA3 depletion decreased an expression of nuclear factor kappa B NFκB targets cyclin D1 and Bcl2 expression as well as IκB phosphorylation Luciferase reporter assay showed that CARMA3 depletion could downregulate NFκB reporter activity In conclusion CARMA3 is overexpressed in bladder cancer and regulates malignant cell growth and NFκB signaling which makes CARMA3 a candidate therapeutic target for bladder cancer
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