Authors: Zhanzhao Fu Guogui Sun Tao Gu
Publish Date: 2013/11/23
Volume: 35, Issue: 4, Pages: 2975-2981
Abstract
This study aimed to analyze the expression clinical significance of protooncogene in non small cell lung cancer NSCLC and the biological effect in its cell line by siRNA targeting wildtype p53induced phosphatase 1 Wip1 Immunohistochemistry and reverse transcription polymerase chain reaction RTPCR were respectively used to analyze Wip1 protein expression in 75 cases of NSCLC and normal tissues to study the relationship between Wip1 expression and clinical parameters Wip1 siRNA was transiently transfected into papillary NSCLC H1299 cell by liposomemediated method and was detected by RTPCR and Western blot MTT assay cell apoptosis and cell cycle were also conducted as to the influence of the downregulated expression of Wip1 that might be found on H1299 cells biological effect The positive rates of Wip1 protein was 693 in NSCLC tissues but 160 expressed in normal tissues P 005 The relative content of Wip1 mRNA was 0785 ± 0062 and 0147 ± 0020 in NSCLC tissues and normal tissues respectively with significant differences between the two types P 005 There were no significant differences between Wip1 expression and sex age tumor size and pathological types P 005 However there were significant differences between Wip1 expression and lymph node metastasis clinical stages and tumor differentiation P 005 Individuals with positive and negative levels of Wip1 expression showed were statistically significant differences in the 5year overall survival rate P 005 RTPCR and Western blot showed that H1299 cell transfected Wip1 siRNA had a lower relative expressive content than normal cell P 005 MTT assay cell apoptosis and cell cycles demonstrated that H1299 cell transfected Wip1 siRNA had a lower survival fraction higher cell apoptosis more percentage of the G0/G1 phases and lower cells in the S phases P 005 Wip1 protein and mRNA were increased in NSCLC specifically in lymph node metastasis clinical stages and tumor differentiation Wip1 may be involved in the biological processes of NSCLC cell proliferation cell apoptosis and cell cycle
Keywords: